The present study describes a simple, accurate, precise and cost effective visible-spectrophotometric method for the estimation of atazanavir sulphate, an anti-HIV drug, in bulk and pharmaceutical dosage form. The stock solution of atazanavir sulphate was prepared by using methanol as solvent. This method based on the complex formation when the atazanavir sulphate was treated with FC (folin ciocalteu) reagent diluted with water (1:3) in presence 20% Na2CO3 solution. The complex is bluish green in colour and has the maximum absorbance at 734 nm. The developed method obeys the beer’s law in the concentration range of 15-75 μg/ml. The method was validated for different parameters as per the ICH (International Conference for harmonization) guidelines. This method can be used for the determination of atazanavir sulphate in quality control of formulation without interference of the excipients.
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